Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [5]
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Validation data
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- Product number
- GTX110607 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX110607, RRID:AB_1950229
- Product name
- Estrogen Receptor beta antibody [N2C2], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Storage
- Keep as concentrated solution. Aliquot and store at -20?C or below. Avoid multiple freeze-thaw cycles.
Submitted references A Ternary Mixture of Common Chemicals Perturbs Benign Human Breast Epithelial Cells More Than the Same Chemicals Do Individually.
Nucleoporin 153 regulates estrogen-dependent nuclear translocation of endothelial nitric oxide synthase and estrogen receptor beta in prostate cancer.
Infiltrating neutrophils promote renal cell carcinoma progression via VEGFa/HIF2α and estrogen receptor β signals.
1,25-Dihydroxyvitamin D3 increases testosterone-induced 17beta-estradiol secretion and reverses testosterone-reduced connexin 43 in rat granulosa cells.
Suppression of ERβ signaling via ERβ knockout or antagonist protects against bladder cancer development.
SGK3 is an androgen-inducible kinase promoting prostate cancer cell proliferation through activation of p70 S6 kinase and up-regulation of cyclin D1.
Activation of the mTOR pathway by low levels of xenoestrogens in breast epithelial cells from high-risk women.
Estrogen receptor beta inhibits transcriptional activity of hypoxia inducible factor-1 through the downregulation of arylhydrocarbon receptor nuclear translocator.
Silencing of GSTP1, a prostate cancer prognostic gene, by the estrogen receptor-β and endothelial nitric oxide synthase complex.
Estrogen receptor β represses Akt signaling in breast cancer cells via downregulation of HER2/HER3 and upregulation of PTEN: implications for tamoxifen sensitivity.
Dairkee SH, Luciani-Torres G, Moore DH, Jaffee IM, Goodson WH 3rd
Toxicological sciences : an official journal of the Society of Toxicology 2018 Sep 1;165(1):131-144
Toxicological sciences : an official journal of the Society of Toxicology 2018 Sep 1;165(1):131-144
Nucleoporin 153 regulates estrogen-dependent nuclear translocation of endothelial nitric oxide synthase and estrogen receptor beta in prostate cancer.
Re A, Colussi C, Nanni S, Aiello A, Bacci L, Grassi C, Pontecorvi A, Farsetti A
Oncotarget 2018 Jun 15;9(46):27985-27997
Oncotarget 2018 Jun 15;9(46):27985-27997
Infiltrating neutrophils promote renal cell carcinoma progression via VEGFa/HIF2α and estrogen receptor β signals.
Song W, Yeh CR, He D, Wang Y, Xie H, Pang ST, Chang LS, Li L, Yeh S
Oncotarget 2015 Aug 7;6(22):19290-304
Oncotarget 2015 Aug 7;6(22):19290-304
1,25-Dihydroxyvitamin D3 increases testosterone-induced 17beta-estradiol secretion and reverses testosterone-reduced connexin 43 in rat granulosa cells.
Lee CT, Wang JY, Chou KY, Hsu MI
Reproductive biology and endocrinology : RB&E 2014 Sep 20;12:90
Reproductive biology and endocrinology : RB&E 2014 Sep 20;12:90
Suppression of ERβ signaling via ERβ knockout or antagonist protects against bladder cancer development.
Hsu I, Chuang KL, Slavin S, Da J, Lim WX, Pang ST, O'Brien JH, Yeh S
Carcinogenesis 2014 Mar;35(3):651-61
Carcinogenesis 2014 Mar;35(3):651-61
SGK3 is an androgen-inducible kinase promoting prostate cancer cell proliferation through activation of p70 S6 kinase and up-regulation of cyclin D1.
Wang Y, Zhou D, Chen S
Molecular endocrinology (Baltimore, Md.) 2014 Jun;28(6):935-48
Molecular endocrinology (Baltimore, Md.) 2014 Jun;28(6):935-48
Activation of the mTOR pathway by low levels of xenoestrogens in breast epithelial cells from high-risk women.
Goodson WH 3rd, Luciani MG, Sayeed SA, Jaffee IM, Moore DH 2nd, Dairkee SH
Carcinogenesis 2011 Nov;32(11):1724-33
Carcinogenesis 2011 Nov;32(11):1724-33
Estrogen receptor beta inhibits transcriptional activity of hypoxia inducible factor-1 through the downregulation of arylhydrocarbon receptor nuclear translocator.
Lim W, Park Y, Cho J, Park C, Park J, Park YK, Park H, Lee Y
Breast cancer research : BCR 2011 Mar 24;13(2):R32
Breast cancer research : BCR 2011 Mar 24;13(2):R32
Silencing of GSTP1, a prostate cancer prognostic gene, by the estrogen receptor-β and endothelial nitric oxide synthase complex.
Re A, Aiello A, Nanni S, Grasselli A, Benvenuti V, Pantisano V, Strigari L, Colussi C, Ciccone S, Mazzetti AP, Pierconti F, Pinto F, Bassi P, Gallucci M, Sentinelli S, Trimarchi F, Bacchetti S, Pontecorvi A, Lo Bello M, Farsetti A
Molecular endocrinology (Baltimore, Md.) 2011 Dec;25(12):2003-16
Molecular endocrinology (Baltimore, Md.) 2011 Dec;25(12):2003-16
Estrogen receptor β represses Akt signaling in breast cancer cells via downregulation of HER2/HER3 and upregulation of PTEN: implications for tamoxifen sensitivity.
Lindberg K, Helguero LA, Omoto Y, Gustafsson JÅ, Haldosén LA
Breast cancer research : BCR 2011 Apr 14;13(2):R43
Breast cancer research : BCR 2011 Apr 14;13(2):R43
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (60 ?g of whole cell lysate)A: GC1B: GC1 co-transfected ER-beta (NM_207707)12% SDS PAGEGTX110607 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE GTX110607 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A:NIH-3T37.5% SDS PAGE GTX110607 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [N2C2], Internal (GTX110607) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [N2C2], Internal (GTX110607) diluted at 1:1000.