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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX70176 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX70176, RRID:AB_370369
- Product name
- Estrogen Receptor beta antibody [4D2]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG D: SK-N-SH 7.5% SDS PAGE GTX70176 diluted at 1:500
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [4D2] (GTX70176) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using ER beta(GTX70176) antibody at 1:200 dilution.
