GTX70182

antibody from GeneTex

Targeting: ESR2 ER-beta, Erb, NR3A2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

GTX70182

Provider

GeneTex

Proper citation

GeneTex Cat#GTX70182, RRID:AB_370375

Product name

Estrogen Receptor beta antibody [7B10.7]

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

References

Submitted references

Comprehensive assessment of estrogen receptor beta antibodies in cancer cell line models and tissue reveals critical limitations in reagent specificity.

Nelson AW, Groen AJ, Miller JL, Warren AY, Holmes KA, Tarulli GA, Tilley WD, Katzenellenbogen BS, Hawse JR, Gnanapragasam VJ, Carroll JS
Molecular and cellular endocrinology 2017 Jan 15;440:138-150

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Estrogen Receptor-β Modulation of the ERα-p53 Loop Regulating Gene Expression, Proliferation, and Apoptosis in Breast Cancer.

Lu W, Katzenellenbogen BS
Hormones & cancer 2017 Aug;8(4):230-242

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Estrogen receptor β regulates endometriotic cell survival through serum and glucocorticoid-regulated kinase activation.

Monsivais D, Dyson MT, Yin P, Navarro A, Coon JS Th, Pavone ME, Bulun SE
Fertility and sterility 2016 May;105(5):1266-1273

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Preferential estrogen receptor β ligands reduce Bcl-2 expression in hormone-resistant breast cancer cells to increase autophagy.

Ruddy SC, Lau R, Cabrita MA, McGregor C, McKay BC, Murphy LC, Wright JS, Durst T, Pratt MA
Molecular cancer therapeutics 2014 Jul;13(7):1882-93

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ERβ- and prostaglandin E2-regulated pathways integrate cell proliferation via Ras-like and estrogen-regulated growth inhibitor in endometriosis.

Monsivais D, Dyson MT, Yin P, Coon JS, Navarro A, Feng G, Malpani SS, Ono M, Ercan CM, Wei JJ, Pavone ME, Su E, Bulun SE
Molecular endocrinology (Baltimore, Md.) 2014 Aug;28(8):1304-15

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Mechanisms enforcing the estrogen receptor β selectivity of botanical estrogens.

Jiang Y, Gong P, Madak-Erdogan Z, Martin T, Jeyakumar M, Carlson K, Khan I, Smillie TJ, Chittiboyina AG, Rotte SC, Helferich WG, Katzenellenbogen JA, Katzenellenbogen BS
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2013 Nov;27(11):4406-18

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Integrative genomics of gene and metabolic regulation by estrogen receptors α and β, and their coregulators.

Madak-Erdogan Z, Charn TH, Jiang Y, Liu ET, Katzenellenbogen JA, Katzenellenbogen BS
Molecular systems biology 2013 Jun 18;9:676

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Induction of quinone reductase by tamoxifen or DPN protects against mammary tumorigenesis.

Krishnamurthy N, Hu Y, Siedlak S, Doughman YQ, Watanabe M, Montano MM
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2012 Oct;26(10):3993-4002

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Estrogen receptor β causes a G2 cell cycle arrest by inhibiting CDK1 activity through the regulation of cyclin B1, GADD45A, and BTG2.

Paruthiyil S, Cvoro A, Tagliaferri M, Cohen I, Shtivelman E, Leitman DC
Breast cancer research and treatment 2011 Oct;129(3):777-84

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Estrogen receptor beta binds to and regulates three distinct classes of target genes.

Vivar OI, Zhao X, Saunier EF, Griffin C, Mayba OS, Tagliaferri M, Cohen I, Speed TP, Leitman DC
The Journal of biological chemistry 2010 Jul 16;285(29):22059-66

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Genome-wide dynamics of chromatin binding of estrogen receptors alpha and beta: mutual restriction and competitive site selection.

Charn TH, Liu ET, Chang EC, Lee YK, Katzenellenbogen JA, Katzenellenbogen BS
Molecular endocrinology (Baltimore, Md.) 2010 Jan;24(1):47-59

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Selective activation of estrogen receptor-beta target genes by 3,3'-diindolylmethane.

Vivar OI, Saunier EF, Leitman DC, Firestone GL, Bjeldanes LF
Endocrinology 2010 Apr;151(4):1662-7

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Estrogen Receptors alpha and beta as determinants of gene expression: influence of ligand, dose, and chromatin binding.

Chang EC, Charn TH, Park SH, Helferich WG, Komm B, Katzenellenbogen JA, Katzenellenbogen BS
Molecular endocrinology (Baltimore, Md.) 2008 May;22(5):1032-43

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Selective activation of estrogen receptor-beta transcriptional pathways by an herbal extract.

Cvoro A, Paruthiyil S, Jones JO, Tzagarakis-Foster C, Clegg NJ, Tatomer D, Medina RT, Tagliaferri M, Schaufele F, Scanlan TS, Diamond MI, Cohen I, Leitman DC
Endocrinology 2007 Feb;148(2):538-47

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Distinct roles of unliganded and liganded estrogen receptors in transcriptional repression.

Cvoro A, Tzagarakis-Foster C, Tatomer D, Paruthiyil S, Fox MS, Leitman DC
Molecular cell 2006 Feb 17;21(4):555-64

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Estradiol and selective estrogen receptor modulators differentially regulate target genes with estrogen receptors alpha and beta.

Tee MK, Rogatsky I, Tzagarakis-Foster C, Cvoro A, An J, Christy RJ, Yamamoto KR, Leitman DC
Molecular biology of the cell 2004 Mar;15(3):1262-72

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Functional implications of antiestrogen induction of quinone reductase: inhibition of estrogen-induced deoxyribonucleic acid damage.

Bianco NR, Perry G, Smith MA, Templeton DJ, Montano MM
Molecular endocrinology (Baltimore, Md.) 2003 Jul;17(7):1344-55

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Reciprocal recruitment of DRIP/mediator and p160 coactivator complexes in vivo by estrogen receptor.

Burakov D, Crofts LA, Chang CP, Freedman LP
The Journal of biological chemistry 2002 Apr 26;277(17):14359-62

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [7B10.7] (GTX70182) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.