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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB12682 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB12682, RRID:AB_10553414
- Product name
- ESR2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ESR2.
- Storage
- Store at 4°C. For long term storage store at -20°C or -80°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Estrogen receptor (ER) beta, a modulator of ERalpha in the uterus.
Weihua Z, Saji S, Mäkinen S, Cheng G, Jensen EV, Warner M, Gustafsson JA
Proceedings of the National Academy of Sciences of the United States of America 2000 May 23;97(11):5936-41
Proceedings of the National Academy of Sciences of the United States of America 2000 May 23;97(11):5936-41
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The whole cell lysates derived from MCF-7 were immunoblotted by ESR2 polyclonal antibody (Cat # PAB12682) at 1 : 500 (Lane 1). The immunoreactive band is blocked by pre-incubation of immunizing peptide (Lane 2).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human breast cancer tissue stained with ESR2 polyclonal antibody (Cat # PAB12682) at 1 : 200 for 10 min at RT. Staining of formalin-fixedtissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
