Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP2-61944 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal ER beta/NR3A2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human, Rat, Chicken/Avian, Porcine, Sheep, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ER beta/NR3A2 Antibody ([PPG5/10]) - Azide and BSA Free [NBP2-61944] - Clone PPG5/10 used to detect expression of ER beta/NR3A2 in cancer tissue by Western Blot. (B) Western blot of ER beta detection in villous trophoblast cell lysate. Lane 1: normal placenta; Lane 2: preeclamptic placenta; Lane 3: IUGR placenta.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ER beta/NR3A2 Antibody ([PPG5/10]) - Azide and BSA Free [NBP2-61944] - Clone PPG5/10 used to detect expression of ER beta/NR3A2 in colorectal tissue by Western Blot. (A) ER beta/NR3A2, 2 and 5 mRNA expression (assessed by RT-PCR) and (B) ER beta/NR3A2 and ER beta 2 protein expression (assessed by western blotting) by 10 colorectal carcinoma cell lines.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: ER beta/NR3A2 Antibody ([PPG5/10]) - Azide and BSA Free [NBP2-61944] - Clone PPG5/10 used to detect expression of ER beta/NR3A2 in endometrial cancer tissue by IHC-P. Tissues were classified as well (A-D), moderately (E-H) or poorly (J-M) differentiated; main panels show closely adjacent sections from three cancer blocks to allow direct comparisons.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: ER beta/NR3A2 Antibody ([PPG5/10]) - Azide and BSA Free [NBP2-61944] - Clone PPG5/10 used to detect expression of ER beta/NR3A2 in cancer tissue by IHC-P. Prostate carcinoma Gleason score 3+3 showing > 70% positive nuclei stained by IHC.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: ER beta/NR3A2 Antibody ([PPG5/10]) - Azide and BSA Free [NBP2-61944] - Clone PPG5/10 used to detect expression of ER beta/NR3A2 in cancer tissue by IHC-P.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: ER beta/NR3A2 Antibody ([PPG5/10]) - Azide and BSA Free [NBP2-61944] - Clone PPG5/10 used to detect expression of ER beta/NR3A2 in cancer tissue by IHC-P. Expression of estrogen receptor (ER) beta (indicated by arrows) in normal syncytiotrophoblasts preeclamptic (A), syncytiotrophoblast (B), and intrauterine growth restriction (C). Expression of ER beta/NR3A2 was also detected in extravillous trophoblast cells of normal-term placentas (D), preeclamptic placentas (E), and intrauterine growth-restricted placentas (F); 25x lens.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: ER beta/NR3A2 Antibody ([PPG5/10]) - Azide and BSA Free [NBP2-61944] - Clone PPG5/10 used to detect expression of ER beta/NR3A2 in cancer tissue by Western Blot. Examples of ER beta/NR3A2 (a, d, e), ER beta 2 (b, f, g), ER beta/NR3A2 (c, h) and TFF1 (i) immunohistochemistry performed on normal colorectal epithelium (a-c) and colorectal carcinoma (d-i).