MA1-23217

antibody from Invitrogen Antibodies

Targeting: ESR2 ER-beta, Erb, NR3A2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Chromatin Immunoprecipitation (Recommended by provider)

Other assay (Recommended by provider)

Antibody Data

Product number

MA1-23217

Provider

Invitrogen Antibodies

Product name

Estrogen Receptor beta Monoclonal Antibody (14C8)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

For immunostaining, strong ER beta staining in epithelial cell nuclei is observed, with weak to moderate staining seen in surrounding stromal and endothelial cell nuclei, and sporadic light cytoplasmic staining. It has also been reported that this antibody has been used to detect ERb in colon and ovarian tumors. Positive control of recombinant fusion protein.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

14C8

Vial size

100 µL

Concentration

1.23 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Effects of Pubertal Exposure to Dietary Soy on Estrogen Receptor Activity in the Breast of Cynomolgus Macaques.

Dewi FN, Wood CE, Willson CJ, Register TC, Lees CJ, Howard TD, Huang Z, Murphy SK, Tooze JA, Chou JW, Miller LD, Cline JM
Cancer prevention research (Philadelphia, Pa.) 2016 May;9(5):385-95

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Estradiol stimulates transcription of nuclear respiratory factor-1 and increases mitochondrial biogenesis.

Mattingly KA, Ivanova MM, Riggs KA, Wickramasinghe NS, Barch MJ, Klinge CM
Molecular endocrinology (Baltimore, Md.) 2008 Mar;22(3):609-22

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of hEstrogen Receptor beta in Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg). Samples were separated by 7.5% SDS-PAGE and the membrane was probed with Estrogen Receptor beta Monoclonal antibody (Product # MA1-23217) at a dilution of 1:5000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Estrogen Receptor beta was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Estrogen Receptor beta Monoclonal Antibody (14C8) (Product # MA1-23217) at a dilution of 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of Estrogen Receptor beta was performed in paraffin-embedded human breast carcinoma tissue using Estrogen Receptor beta Monoclonal Antibody (14C8) (Product # MA1-23217) at a dilution of 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of Infiltrating lobular carcinoma of the breast using Estrogen Receptor beta Monoclonal Antibody (14C8) (Product # MA1-23217).