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LearnNB200-303
antibody from Novus Biologicals
Targeting: ESR2
ER-beta, Erb, NR3A2
Western blot
Immunocytochemistry Immunoprecipitation Immunohistochemistry Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [16]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- NB200-303 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB200-303, RRID:AB_10128112
- Product name
- Mouse Monoclonal ER beta/NR3A2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This is specific for Estrogen receptor-Beta.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Sex steroid metabolism and actions in non-small cell lung carcinoma.
Estrogen receptor beta binds to and regulates three distinct classes of target genes.
Selective activation of estrogen receptor-beta target genes by 3,3'-diindolylmethane.
Activity and intracellular location of estrogen receptors alpha and beta in human bronchial epithelial cells.
Resveratrol stimulates nitric oxide production by increasing estrogen receptor alpha-Src-caveolin-1 interaction and phosphorylation in human umbilical vein endothelial cells.
Selective activation of estrogen receptor-beta transcriptional pathways by an herbal extract.
Distinct roles of unliganded and liganded estrogen receptors in transcriptional repression.
Low levels of estrogen receptor beta protein predict resistance to tamoxifen therapy in breast cancer.
Estradiol and selective estrogen receptor modulators differentially regulate target genes with estrogen receptors alpha and beta.
Oestrogen receptor beta (ERbeta) is abundantly expressed in normal colonic mucosa, but declines in colon adenocarcinoma paralleling the tumour's dedifferentiation.
Oestrogen receptor beta (ERbeta) is abundantly expressed in normal colonic mucosa, but declines in colon adenocarcinoma paralleling the tumour's dedifferentiation.
Functional implications of antiestrogen induction of quinone reductase: inhibition of estrogen-induced deoxyribonucleic acid damage.
Correspondence re: S. A. W. Fuqua et al., Estrogen receptor beta protein in human breast cancer: correlation with clinical tumor parameters. Cancer Res.,63: 2434-2439, 2003.
Expression of estrogen receptor (ER) (beta)cx protein in ER(alpha)-positive breast cancer: specific correlation with progesterone receptor.
Distinct expression patterns of ER alpha and ER beta in normal human mammary gland.
Distinct expression patterns of ER alpha and ER beta in normal human mammary gland.
Asavasupreechar T, Chan MSM, Saito R, Miki Y, Boonyaratanakornkit V, Sasano H
The Journal of steroid biochemistry and molecular biology 2019 Oct;193:105440
The Journal of steroid biochemistry and molecular biology 2019 Oct;193:105440
Estrogen receptor beta binds to and regulates three distinct classes of target genes.
Vivar OI, Zhao X, Saunier EF, Griffin C, Mayba OS, Tagliaferri M, Cohen I, Speed TP, Leitman DC
The Journal of biological chemistry 2010 Jul 16;285(29):22059-66
The Journal of biological chemistry 2010 Jul 16;285(29):22059-66
Selective activation of estrogen receptor-beta target genes by 3,3'-diindolylmethane.
Vivar OI, Saunier EF, Leitman DC, Firestone GL, Bjeldanes LF
Endocrinology 2010 Apr;151(4):1662-7
Endocrinology 2010 Apr;151(4):1662-7
Activity and intracellular location of estrogen receptors alpha and beta in human bronchial epithelial cells.
Ivanova MM, Mazhawidza W, Dougherty SM, Minna JD, Klinge CM
Molecular and cellular endocrinology 2009 Jun 16;305(1-2):12-21
Molecular and cellular endocrinology 2009 Jun 16;305(1-2):12-21
Resveratrol stimulates nitric oxide production by increasing estrogen receptor alpha-Src-caveolin-1 interaction and phosphorylation in human umbilical vein endothelial cells.
Klinge CM, Wickramasinghe NS, Ivanova MM, Dougherty SM
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Jul;22(7):2185-97
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Jul;22(7):2185-97
Selective activation of estrogen receptor-beta transcriptional pathways by an herbal extract.
Cvoro A, Paruthiyil S, Jones JO, Tzagarakis-Foster C, Clegg NJ, Tatomer D, Medina RT, Tagliaferri M, Schaufele F, Scanlan TS, Diamond MI, Cohen I, Leitman DC
Endocrinology 2007 Feb;148(2):538-47
Endocrinology 2007 Feb;148(2):538-47
Distinct roles of unliganded and liganded estrogen receptors in transcriptional repression.
Cvoro A, Tzagarakis-Foster C, Tatomer D, Paruthiyil S, Fox MS, Leitman DC
Molecular cell 2006 Feb 17;21(4):555-64
Molecular cell 2006 Feb 17;21(4):555-64
Low levels of estrogen receptor beta protein predict resistance to tamoxifen therapy in breast cancer.
Hopp TA, Weiss HL, Parra IS, Cui Y, Osborne CK, Fuqua SA
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Nov 15;10(22):7490-9
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Nov 15;10(22):7490-9
Estradiol and selective estrogen receptor modulators differentially regulate target genes with estrogen receptors alpha and beta.
Tee MK, Rogatsky I, Tzagarakis-Foster C, Cvoro A, An J, Christy RJ, Yamamoto KR, Leitman DC
Molecular biology of the cell 2004 Mar;15(3):1262-72
Molecular biology of the cell 2004 Mar;15(3):1262-72
Oestrogen receptor beta (ERbeta) is abundantly expressed in normal colonic mucosa, but declines in colon adenocarcinoma paralleling the tumour's dedifferentiation.
Konstantinopoulos PA, Kominea A, Vandoros G, Sykiotis GP, Andricopoulos P, Varakis I, Sotiropoulou-Bonikou G, Papavassiliou AG
European journal of cancer (Oxford, England : 1990) 2003 Jun;39(9):1251-8
European journal of cancer (Oxford, England : 1990) 2003 Jun;39(9):1251-8
Oestrogen receptor beta (ERbeta) is abundantly expressed in normal colonic mucosa, but declines in colon adenocarcinoma paralleling the tumour's dedifferentiation.
Konstantinopoulos PA, Kominea A, Vandoros G, Sykiotis GP, Andricopoulos P, Varakis I, Sotiropoulou-Bonikou G, Papavassiliou AG
European journal of cancer (Oxford, England : 1990) 2003 Jun;39(9):1251-8
European journal of cancer (Oxford, England : 1990) 2003 Jun;39(9):1251-8
Functional implications of antiestrogen induction of quinone reductase: inhibition of estrogen-induced deoxyribonucleic acid damage.
Bianco NR, Perry G, Smith MA, Templeton DJ, Montano MM
Molecular endocrinology (Baltimore, Md.) 2003 Jul;17(7):1344-55
Molecular endocrinology (Baltimore, Md.) 2003 Jul;17(7):1344-55
Correspondence re: S. A. W. Fuqua et al., Estrogen receptor beta protein in human breast cancer: correlation with clinical tumor parameters. Cancer Res.,63: 2434-2439, 2003.
Speirs V
Cancer research 2003 Dec 15;63(24):9055
Cancer research 2003 Dec 15;63(24):9055
Expression of estrogen receptor (ER) (beta)cx protein in ER(alpha)-positive breast cancer: specific correlation with progesterone receptor.
Saji S, Omoto Y, Shimizu C, Warner M, Hayashi Y, Horiguchi S, Watanabe T, Hayashi S, Gustafsson JA, Toi M
Cancer research 2002 Sep 1;62(17):4849-53
Cancer research 2002 Sep 1;62(17):4849-53
Distinct expression patterns of ER alpha and ER beta in normal human mammary gland.
Speirs V, Skliris GP, Burdall SE, Carder PJ
Journal of clinical pathology 2002 May;55(5):371-4
Journal of clinical pathology 2002 May;55(5):371-4
Distinct expression patterns of ER alpha and ER beta in normal human mammary gland.
Speirs V, Skliris GP, Burdall SE, Carder PJ
Journal of clinical pathology 2002 May;55(5):371-4
Journal of clinical pathology 2002 May;55(5):371-4
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ER beta/NR3A2 Antibody (6A12) [NB200-303] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [6A12]. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
