MAB7106

antibody from R&D Systems

Targeting: ESR2 ER-beta, Erb, NR3A2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB7106

Provider

R&D Systems

Product name

Human ER beta/NR3A2 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human ER beta/NR3A2 in direct ELISAs and Western blot. In direct ELISA and Western blot, no cross-reactivity with recombinant human ER alpha is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

Q92731


Isotype

IgG

Antibody clone number

733930

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Estradiol-mediated improvements in adipose tissue insulin sensitivity are related to the balance of adipose tissue estrogen receptor α and β in postmenopausal women.

Park YM, Pereira RI, Erickson CB, Swibas TA, Cox-York KA, Van Pelt RE
PloS one 2017;12(5):e0176446

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human ER beta/NR3A2 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, Saos-2 human osteosarcoma cell line, and T47D human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ER beta/NR3A2 Monoclonal Antibody (Catalog # MAB7106) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ER beta/NR3A2 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

ER beta/NR3A2 in LNCaP Human Cell Line. ER beta/NR3A2 was detected in immersion fixed LNCaP human prostate cancer cell line using Mouse Anti-Human ER beta/NR3A2 Monoclonal Antibody (Catalog # MAB7106) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

ER beta/NR3A2 in MCF-7 Human Cell Line. ER beta/NR3A2 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human ER beta/NR3A2 Monoclonal Antibody (Catalog # MAB7106) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of ER beta/NR3A2 in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human ER beta/NR3A2 Monoclonal Antibody (Catalog # MAB7106, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.