Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 12-2589-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD258 (LIGHT) Monoclonal Antibody (7-3 (7)), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 7-3 (7) monoclonal antibody reacts with human CD258 (also known as LIGHT), a 29-kDa type II transmembrane protein that is a member of the TNF ligand superfamily related to lymphotoxin-β (LT-β). Expressed on activated T cells, particularly CD8+ T cells, LIGHT plays a role in cell survival, inflammation, and tumor eradication. Furthermore, upon binding its ligand, herpesvirus entry mediator (HVEM), LIGHT induces a costimulatory effect on T cells, enhancing T cell proliferation and cytokine production. LIGHT has also been shown to indirectly interact with the LT-β receptor.
- Conjugate
- Yellow dye
- Antibody clone number
- 7-3 (7)
- Concentration
- 5 µL/Test
Submitted references TIGIT blockade enhances functionality of peritoneal NK cells with altered expression of DNAM-1/TIGIT/CD96 checkpoint molecules in ovarian cancer.
PD-1 Blockade Promotes Emerging Checkpoint Inhibitors in Enhancing T Cell Responses to Allogeneic Dendritic Cells.
LIGHT expression by mucosal T cells may regulate IFN-gamma expression in the intestine.
Direct fluorescent labeling of cells with fluorescein or rhodamine isothiocyanate. I. Technical aspects.
Maas RJ, Hoogstad-van Evert JS, Van der Meer JM, Mekers V, Rezaeifard S, Korman AJ, de Jonge PK, Cany J, Woestenenk R, Schaap NP, Massuger LF, Jansen JH, Hobo W, Dolstra H
Oncoimmunology 2020 Nov 8;9(1):1843247
Oncoimmunology 2020 Nov 8;9(1):1843247
PD-1 Blockade Promotes Emerging Checkpoint Inhibitors in Enhancing T Cell Responses to Allogeneic Dendritic Cells.
Stecher C, Battin C, Leitner J, Zettl M, Grabmeier-Pfistershammer K, Höller C, Zlabinger GJ, Steinberger P
Frontiers in immunology 2017;8:572
Frontiers in immunology 2017;8:572
LIGHT expression by mucosal T cells may regulate IFN-gamma expression in the intestine.
Cohavy O, Zhou J, Granger SW, Ware CF, Targan SR
Journal of immunology (Baltimore, Md. : 1950) 2004 Jul 1;173(1):251-8
Journal of immunology (Baltimore, Md. : 1950) 2004 Jul 1;173(1):251-8
Direct fluorescent labeling of cells with fluorescein or rhodamine isothiocyanate. I. Technical aspects.
Butcher EC, Weissman IL
Journal of immunological methods 1980;37(2):97-108
Journal of immunological methods 1980;37(2):97-108
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of PMA and Ionomycin stimulated normal human peripheral blood cells with Anti-Human CD8a APC (Product # 17-0088-42) and Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD258 (LIGHT) PE (right). Total viable cells were used for analysis.
- Conjugate
- Yellow dye