12-2589-42

antibody from Invitrogen Antibodies
Targeting: TNFSF14 CD258, HVEM-L, LIGHT, LTg
Provider product page for 12-2589-42

Antibody data

Product number
12-2589-42 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD258 (LIGHT) Monoclonal Antibody (7-3 (7)), PE, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: This 7-3 (7) monoclonal antibody reacts with human CD258 (also known as LIGHT), a 29-kDa type II transmembrane protein that is a member of the TNF ligand superfamily related to lymphotoxin-β (LT-β). Expressed on activated T cells, particularly CD8+ T cells, LIGHT plays a role in cell survival, inflammation, and tumor eradication. Furthermore, upon binding its ligand, herpesvirus entry mediator (HVEM), LIGHT induces a costimulatory effect on T cells, enhancing T cell proliferation and cytokine production. LIGHT has also been shown to indirectly interact with the LT-β receptor. Applications Reported: This 7-3 (7) antibody has been reported for use in flow cytometric analysis. Applications Tested: This 7-3 (7) antibody has been pre-titrated and tested by flow cytometric analysis on PMA- and ionomycin-stimulated normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Conjugate
Yellow dye
Isotype
IgG
Antibody clone number
7-3 (7)
Vial size
100 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
TNFSF14 protein structure - 12-2589-42 shown in red.