Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 17-2589-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD258 (LIGHT) Monoclonal Antibody (7-3 (7)), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 7-3 (7) monoclonal antibody reacts with human CD258 (also known as LIGHT), a 29-kDa type II transmembrane protein that is a member of the TNF ligand superfamily related to lymphotoxin-β (LT-β). Expressed on activated T cells, particularly CD8+ T cells, LIGHT plays a role in cell survival, inflammation, and tumor eradication. Furthermore, upon binding its ligand, herpesvirus entry mediator (HVEM), LIGHT induces a costimulatory effect on T cells, enhancing T cell proliferation and cytokine production. LIGHT has also been shown to indirectly interact with the LT-β receptor. Applications Reported: This 7-3 (7) antibody has been reported for use in flow cytometric analysis. Applications Tested: This 7-3 (7) antibody has been tested by flow cytometric analysis on PMA- and ionomycin-stimulated normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 7-3 (7)
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references LIGHT expression by mucosal T cells may regulate IFN-gamma expression in the intestine.
Cohavy O, Zhou J, Granger SW, Ware CF, Targan SR
Journal of immunology (Baltimore, Md. : 1950) 2004 Jul 1;173(1):251-8
Journal of immunology (Baltimore, Md. : 1950) 2004 Jul 1;173(1):251-8
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of PMA/Ionomycin-stimulated normal human peripheral blood cells with Anti-Human CD8a PE (Product # 12-0088-80) and Mouse IgG1 kappa Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD258 (LIGHT) APC (right). Total viable cells were used for analysis.