Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- ELISA [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF664 - Provider product page
- Provider
- R&D Systems
- Product name
- Human LIGHT/TNFSF14 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human LIGHT/TNFSF14 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) APRIL is observed and less than 1% cross-reactivity with rhFas Ligand, rhTRAIL, and rhTNF-alpha is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O43557
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Licensed human natural killer cells aid dendritic cell maturation via TNFSF14/LIGHT.
VEGF-C is a trophic factor for neural progenitors in the vertebrate embryonic brain.
Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes.
Holmes TD, Wilson EB, Black EV, Benest AV, Vaz C, Tan B, Tanavde VM, Cook GP
Proceedings of the National Academy of Sciences of the United States of America 2014 Dec 30;111(52):E5688-96
Proceedings of the National Academy of Sciences of the United States of America 2014 Dec 30;111(52):E5688-96
VEGF-C is a trophic factor for neural progenitors in the vertebrate embryonic brain.
Le Bras B, Barallobre MJ, Homman-Ludiye J, Ny A, Wyns S, Tammela T, Haiko P, Karkkainen MJ, Yuan L, Muriel MP, Chatzopoulou E, Bréant C, Zalc B, Carmeliet P, Alitalo K, Eichmann A, Thomas JL
Nature neuroscience 2006 Mar;9(3):340-8
Nature neuroscience 2006 Mar;9(3):340-8
Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes.
Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damås JK
Blood 2006 Aug 1;108(3):928-35
Blood 2006 Aug 1;108(3):928-35
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Human LIGHT/TNFSF14 ELISA Standard Curve. Recombinant Human LIGHT/TNFSF14 protein was serially diluted 2-fold and captured by Mouse Anti-Human LIGHT/TNFSF14 Monoclonal Antibody (Catalog # MAB6642) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human LIGHT/TNFSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF664) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- LIGHT/TNFSF14 in Human Spleen. LIGHT/TNFSF14 was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human LIGHT/TNFSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF664) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to splenocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by LIGHT/TNFSF14 and Neutral-ization by Human LIGHT/ TNFSF14 Antibody. Recombinant Human LIGHT/ TNFSF14 (Catalog # 664-LI) induces proliferation in the HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human LIGHT/ TNFSF14 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human LIGHT/ TNFSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF664). The ND50 is typically 5-20 ng/mL.