Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- FAB664A - Provider product page
- Provider
- R&D Systems
- Product name
- Human LIGHT/TNFSF14 APC-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human LIGHT/TNFSF14 in ELISAs. In sandwich immunoassays, no significant cross-reactivity or interference with recombinant human (rh) Fas Ligand, rhAPRIL, recombinant mouse TRANCE, rhTNF-alpha or rhTRAIL is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Red dye
- Antigen sequence
O43557
- Isotype
- IgG
- Antibody clone number
- 115520
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Licensed human natural killer cells aid dendritic cell maturation via TNFSF14/LIGHT.
Holmes TD, Wilson EB, Black EV, Benest AV, Vaz C, Tan B, Tanavde VM, Cook GP
Proceedings of the National Academy of Sciences of the United States of America 2014 Dec 30;111(52):E5688-96
Proceedings of the National Academy of Sciences of the United States of America 2014 Dec 30;111(52):E5688-96
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of LIGHT/TNFSF14 in Human CD3+ T cells by Flow Cytometry. Human CD3+ T cells treated with 1 ng/mL PMA and Calcium Ionomycin for 36 hours were stained with Mouse Anti-Human LIGHT/TNFSF14 APC-conjugated Monoclonal Antibody (Catalog # FAB664A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). Cells were gate on CD4+ cells using Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P). View our protocol for Staining Membrane-associated Proteins.