Explore
Validate
Learn
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- FAB664P - Provider product page
- Provider
- R&D Systems
- Product name
- Human LIGHT/TNFSF14 PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human LIGHT/TNFSF14 in ELISAs. In sandwich immunoassays, no significant cross-reactivity or interference with recombinant human (rh) Fas Ligand, rhAPRIL, recombinant mouse TRANCE, rhTNF-alpha or rhTRAIL is observed.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
O43557- Isotype
- IgG
- Antibody clone number
- 115520
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Licensed human natural killer cells aid dendritic cell maturation via TNFSF14/LIGHT.
DcR3 binds to ovarian cancer via heparan sulfate proteoglycans and modulates tumor cells response to platinum with corresponding alteration in the expression of BRCA1.
Platelet-associated LIGHT (TNFSF14) mediates adhesion of platelets to human vascular endothelium.
Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes.
Holmes TD, Wilson EB, Black EV, Benest AV, Vaz C, Tan B, Tanavde VM, Cook GP
Proceedings of the National Academy of Sciences of the United States of America 2014 Dec 30;111(52):E5688-96
Proceedings of the National Academy of Sciences of the United States of America 2014 Dec 30;111(52):E5688-96
DcR3 binds to ovarian cancer via heparan sulfate proteoglycans and modulates tumor cells response to platinum with corresponding alteration in the expression of BRCA1.
Connor JP, Felder M, Kapur A, Onujiogu N
BMC cancer 2012 May 14;12:176
BMC cancer 2012 May 14;12:176
Platelet-associated LIGHT (TNFSF14) mediates adhesion of platelets to human vascular endothelium.
Celik S, Langer H, Stellos K, May AE, Shankar V, Kurz K, Katus HA, Gawaz MP, Dengler TJ
Thrombosis and haemostasis 2007 Oct;98(4):798-805
Thrombosis and haemostasis 2007 Oct;98(4):798-805
Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes.
Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damås JK
Blood 2006 Aug 1;108(3):928-35
Blood 2006 Aug 1;108(3):928-35
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of LIGHT/TNFSF14 in Human CD3+ T cells by Flow Cytometry. Human CD3+ T cells treated with 1 ng/mL PMA and 1 μg/mL Calcium Ionomycin for 36 hours were stained with Mouse Anti-Human LIGHT/TNFSF14 PE-conjugated Monoclonal Antibody (Catalog # FAB664P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). Cells were gated on CD4+ cells using Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A). View our protocol for Staining Membrane-associated Proteins.
