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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- TA319342 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal APC6 phospho T580 antibody (Phospho-specific)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal APC6 phospho T580 antibody (Phospho-specific)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- CDC16
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 0.97 mg/mL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB using Anti-APC6 pT580 antibody shows detection of a band ~72 kDa corresponding to phosphorylated human APC6 (arrowhead lane 1). Lane 1 - nocodazole treated HeLa whole cell lysate. Lane 2 - Reactivity is not seen in lysates from asynchronous HeLa whole cell cultures. Each lane contains approximately 35ug of lysates. Primary antibody diluted to 1:1,000 overnight at 4°C followed by washes and reaction with a 1:10,000 dilution of IRDye?800 conjugated Gt-a-Rabbit IgG [H&L] MX .
- Validation comment
- WB
