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- Antibody Data
- Antigen structure
- References [3]
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- Western blot [1]
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- Product number
- PAB10325 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10325, RRID:AB_1676130
- Product name
- CDC16 (phospho T580) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDC16.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Mammalian p55CDC mediates association of the spindle checkpoint protein Mad2 with the cyclosome/anaphase-promoting complex, and is involved in regulating anaphase onset and late mitotic events.
The serine/threonine phosphatase PP5 interacts with CDC16 and CDC27, two tetratricopeptide repeat-containing subunits of the anaphase-promoting complex.
CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition.
Kallio M, Weinstein J, Daum JR, Burke DJ, Gorbsky GJ
The Journal of cell biology 1998 Jun 15;141(6):1393-406
The Journal of cell biology 1998 Jun 15;141(6):1393-406
The serine/threonine phosphatase PP5 interacts with CDC16 and CDC27, two tetratricopeptide repeat-containing subunits of the anaphase-promoting complex.
Ollendorff V, Donoghue DJ
The Journal of biological chemistry 1997 Dec 19;272(51):32011-8
The Journal of biological chemistry 1997 Dec 19;272(51):32011-8
CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition.
Tugendreich S, Tomkiel J, Earnshaw W, Hieter P
Cell 1995 Apr 21;81(2):261-8
Cell 1995 Apr 21;81(2):261-8
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- Experimental details
- Western blot using CDC16 (phospho T580) polyclonal antibody (Cat # PAB10325) shows detectionof a band ~ 72 KDa corresponding to phosphorylated human CDC16 (arrowhead lane 1).Lane 1 -nocodazole treated HeLa whole cell lysate.Lane 2 -Reactivity is not seen in lysates from asynchronous HeLa whole cell cultures.Each lane contains approximately 35 ug of lysates, separated by 4-20% SDS-PAGE Tris-HEPES and then transferred to nitrocellulose.After blocking the membrane was probed with the primary antibody diluted to 1 : 1,000 overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
