LS-B10410

antibody from LSBio

Targeting: SAG ARRESTIN, RP47

Western blot (Data presented on provider website)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-B10410

Provider

LSBio

Product name

IHC-plus™ SAG / Arrestin Antibody (clone S128) LS-B10410

Antibody type

Monoclonal

Description

Affinity purified

Reactivity

Human, Mouse, Rat, Bovine, Porcine

Host

Mouse

Isotype

IgG

Antibody clone number

S128

Storage

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Confocal image of a pig retina stained with SAG / Arrestin antibody (green). Visual arrestin is most abundant in the outer segments (OS) and inner surface of the outer nuclear layer (ONL), and can be used to identify components of rod photoreceptor cells. (Cone photoreceptors have a different arrestin isotype). Other retinal layers are inner segments (IS), outer plexiform layer (OPL), inner nuclear layer (INL) and inner plexiform layer (IPL). The red stain is Fox2, an RNA binding nuclear protein related to Fox3/NeuN, which stains nuclei of horizontal neurons and some other neurons in the INL and IPL. Nuclear DNA was revealed with DAPI (blue).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-SAG / Arrestin antibody IHC staining of human retina. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Confocal image of a pig retina stained with SAG / Arrestin antibody (green). Visual arrestin is most abundant in the outer segments (OS) and inner surface of the outer nuclear layer (ONL), and can be used to identify components of rod photoreceptor cells. (Cone photoreceptors have a different arrestin isotype). Other retinal layers are inner segments (IS), outer plexiform layer (OPL), inner nuclear layer (INL) and inner plexiform layer (IPL). The red stain is Fox2, an RNA binding nuclear protein related to Fox3/NeuN, which stains nuclei of horizontal neurons and some other neurons in the INL and IPL. Nuclear DNA was revealed with DAPI (blue).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Confocal image of a pig retina stained with SAG / Arrestin antibody (green). Visual arrestin is most abundant in the outer segments (OS) and inner surface of the outer nuclear layer (ONL), and can be used to identify components of rod photoreceptor cells. (Cone photoreceptors have a different arrestin isotype). Other retinal layers are inner segments (IS), outer plexiform layer (OPL), inner nuclear layer (INL) and inner plexiform layer (IPL). The red stain is Fox2, an RNA binding nuclear protein related to Fox3/NeuN, which stains nuclei of horizontal neurons and some other neurons in the INL and IPL. Nuclear DNA was revealed with DAPI (blue).