Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ABIN1580413 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-S-Antigen, Retina and Pineal Gland (Arrestin) (SAG) antibody
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- affinity purified antibody
- Reactivity
- Human, Mouse, Rat, Bovine, Porcine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- S128
- Vial size
- 100 μL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term or -20°C long term.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Phosphodiesterase activation by photoexcited rhodopsin is quenched when rhodopsin is phosphorylated and binds the intrinsic 48-kDa protein of rod outer segments.
Light-induced binding of 48-kDa protein to photoreceptor membranes is highly enhanced by phosphorylation of rhodopsin.
[Isolation and characterization of a retinal antigen inducing experimental autoimmune uveo-retinitis].
Experimental allergic uveitis. Isolation, characterization, and localization of a soluble uveitopathogenic antigen from bovine retina.
Wilden U, Hall SW, Kühn H
Proceedings of the National Academy of Sciences of the United States of America 1986 Mar;83(5):1174-8
Proceedings of the National Academy of Sciences of the United States of America 1986 Mar;83(5):1174-8
Light-induced binding of 48-kDa protein to photoreceptor membranes is highly enhanced by phosphorylation of rhodopsin.
Kühn H, Hall SW, Wilden U
FEBS letters 1984 Oct 29;176(2):473-8
FEBS letters 1984 Oct 29;176(2):473-8
[Isolation and characterization of a retinal antigen inducing experimental autoimmune uveo-retinitis].
Dorey C, Faure JP
Annales d'immunologie 1977 Jan-Mar;128(1-2):229-32
Annales d'immunologie 1977 Jan-Mar;128(1-2):229-32
Experimental allergic uveitis. Isolation, characterization, and localization of a soluble uveitopathogenic antigen from bovine retina.
Wacker WB, Donoso LA, Kalsow CM, Yankeelov JA Jr, Organisciak DT
Journal of immunology (Baltimore, Md. : 1950) 1977 Dec;119(6):1949-58
Journal of immunology (Baltimore, Md. : 1950) 1977 Dec;119(6):1949-58
No comments: Submit comment
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Blot of bovine retinal extracts probed with ABIN1580413. The antibody stains a band corresponding to retinal arrestin at about 48 kDa.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal image of a pig retina stained with ABIN1580413 (green). Visual arrestin is most abundant in the outer segments (OS) and inner surface of the outer nuclear layer (ONL), and can be used to identify components of rod photoreceptor cells. (Cone photoreceptors have a different arrestin isotype). Other retinal layers are inner segments (IS), outer plexiform layer (OPL), inner nuclear layer (INL) and inner plexiform layer (IPL). The red stain is Fox2, an RNA binding nuclear protein related to Fox3/NeuN, which stains nuclei of horizontal neurons and some other neurons in the INL and IPL. Nuclear DNA was revealed with DAPI (blue).
