MA3-814

antibody from Invitrogen Antibodies

Targeting: SAG ARRESTIN, RP47

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA3-814

Provider

Invitrogen Antibodies

Product name

S-arrestin Monoclonal Antibody (PDS1)

Antibody type

Monoclonal

Antigen

Other

Description

MA3-814 detects Visual Arrestin from human, rat, porcine, bovine, ovine, and goat tissues. MA3-814 has been successfully used in Immunohistochemistry, ELISA, and Western blotting procedures. By Western blot it detects a 37-kDa band representing Visual arrestin. The MA3-814 antigen is Visual arrestin isolated from Porcine cells.

Reactivity

Human, Rat, Bovine, Goat, Porcine

Host

Mouse

Isotype

IgG

Antibody clone number

PDS1

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Analysis of antigenic determinants of retinal S-antigen with monoclonal antibodies.

Banga JP, LeRoy F, Suleyman S, Kasp E, Brown E, Dumonde D
Investigative ophthalmology & visual science 1988 Jan;29(1):12-21

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on human retina tissue. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Visual Arrestin mouse monoclonal antibody (Product # MA3-814) at a dilution of 1:20 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.