PA5-97089

antibody from Invitrogen Antibodies

Targeting: PPP4R3A FLFL1, FLJ20707, KIAA2010, MSTP033, PP4R3, SMEK1, smk-1, smk1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-97089

Provider

Invitrogen Antibodies

Product name

SMEK1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: VQTFKGLKLR FEQQRERQDN PKLDSMRSIL RNHRYRRDAR TLEDEEEMWF NTDEDDMEDG EAVVSPSDKT KNDDDIMDPI SKFMERKKLK ESEEKEVLLK TNLSGRQSPS FKLSLSSGTK TNLTSQSSTT NLPGSPGSPG SPGSPGSPGS VPKNTSQTAA ITTKGGLVGL VDYPDDDEDD DEDEDKEDTL PLSKKAKFDS; Positive Samples: HeLa, HepG2, Jurkat, MCF-7, Mouse spleen, Rat brain; Cellular Location: Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.71 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SMEK1 in extracts of various cell lines. Samples were incubated with SMEK1 Polyclonal antibody (Product # PA5-97089) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SMEK1 in paraffin-embedded rat ovary. Samples were incubated with SMEK1 Polyclonal antibody (Product # PA5-97089) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SMEK1 in paraffin-embedded human esophageal cancer. Samples were incubated with SMEK1 Polyclonal antibody (Product # PA5-97089) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SMEK1 in paraffin-embedded mouse kidney. Samples were incubated with SMEK1 Polyclonal antibody (Product # PA5-97089) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.