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LearnA300-840A
antibody from Invitrogen Antibodies
Targeting: PPP4R3A
FLFL1, FLJ20707, KIAA2010, MSTP033, PP4R3, SMEK1, smk-1, smk1
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- A300-840A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PPP4R3 Alpha Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PPP4R3 Alpha by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg) and mouse NIH3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PPP4R3 alpha antibody BL3121 (Cat. No. A300-840A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). PPP4R3 alpha was also immunoprecipitated by rabbit anti-PPP4R3 alpha antibody BL3120 (Cat. No. A300-839A), which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 0.5 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PPP4R3 Alpha by immunohistochemistry. Sample: FFPE sections of human colon carcinoma (left) and breast carcinoma (right). Antibody: Affinity purified rabbit anti- PPP4R3 Alpha (Cat. No. A300-840A Lot1) used at a dilution of 1:200 (1µg/ml) and 1:80 (2.5 µg/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.
