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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP05063PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP05063PU-N, RRID:AB_1624421
- Product name
- anti VAV2 (SHD Region)
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 0.25 mg
- Concentration
- Lot specific
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- HEK293 cells were transfected with either emply expression vector (pSRa) or pSRa-Vav2 driving expression of human Vav2. Indicated antibodies (SHD or DPH) were used to immunoprecipitate Vav2 from 300ug protein lysates. Immunoprecipitated activity was identified by immunoblot of complexes using either DPH (upper gel) or SHD (lower gel) antisera at (1:10000). Immunoprecipitation of Endogenous/Exogenous Vav2 from HEK 283 (75 µg lysate per lane). Top gel - i.p. Ab: DPH or SHD preimmune serum (2.5 µl/300 µg lysate). DPH or SHD alpha Vav2 antiserum (2.5 µl/300 µg lysate). Western Blot Ab: DPH anti Vav2 1:10000. Bottom Gel - i.p. Ab DPH or SHD preimmune sera (2.5 µl/300 µg lysate). DPH or SHD anti Vav2 antisera (2.5 µl/300 µg lysate). Wersterm blot Ab - SHD anti Vav2 antibody 1:10000.
