AP05064PU-N

antibody from Acris Antibodies GmbH

Targeting: VAV2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

AP05064PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP05064PU-N, RRID:AB_1624420

Product name

anti VAV2 (DPH Region)

Antibody type

Polyclonal

Antigen

A his tagged fusion protein Vav-2 corresponding to amino acid sequence 287-578 and having a Molecular Weight of 100kD

Reactivity

Human

Host

Sheep

Isotype

IgG

Vial size

0.25 mg

Concentration

Lot specific

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

HEK293 cells were transfected with either emply expression vector (pSR_) or pSRa-Vav2 driving expression of human Vav2. Indicated antibodies (SHD or DPH) were used to immunoprecipitate Vav2 from 300µg protein lysates. Immunoprecipitated activity was identified by immunoblot of complexes using either DPH (upper gel) or SHD (lower gel) antisera at (1:10000). Immunoprecipitation of Endogenous/Exogenous Vav2 from HEK 283 (75 µg lysate per lane). Top gel - i.p. Ab: DPH or SHD preimmune serum (2.5 µl/300µg lysate). DPH or SHD alpha Vav2 antiserum (2.5 µl/300µg lysate). Western Blot Ab: DPH anti Vav2 1:10000. Bottom Gel - i.p. Ab DPH or SHD preimmune sera (2.5 µl/300µg lysate). DPH or SHD anti Vav2 antisera (2.5µl/300ug lysate). Wersterm blot Ab - SHD anti Vav2 antibody 1:10000.