Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- ABIN306672 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Vav 2 Guanine Nucleotide Exchange Factor (VAV2) antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 250 μg
- Concentration
- 1 mg/ml
- Storage
- -20°C
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Supportive validation
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- antibodies-online (provider)
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- Experimental details
- HEK293 cells were transfected with either emply expression vector (pSR?) or pSRa- Vav2 driving expression of human Vav2. Indicated antibodies (SHD or DPH) were used to immunoprecipitate Vav2 from 300ug protein lysates. Immunoprecipitated activity was identified by immunoblot of complexes using either DPH (upper gel) or SHD (lower gel) antisera at (1:10000). Immunoprecipitation of Endogenous/Exogenous Vav2 from HEK 283 (75 u?g lysate per lane). Top gel - i.p. Ab: DPH or SHD preimmune serum (2.5 ?l/300 ?g lysate). DPH or SHD alpha Vav2 antiserum (2.5 ?l/300 ?g lysate). Western Blot Ab: DPH anti Vav2 1:10000. Bottom Gel - i.p. Ab DPH or SHD preimmune sera (2.5 ?l/300 ?g lysate). DPH or SHD anti Vav2