ABIN306673

antibody from antibodies-online

Targeting: VAV2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN306673

Provider

antibodies-online

Product name

anti-Vav 2 Guanine Nucleotide Exchange Factor (VAV2) antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Sheep

Isotype

IgG

Vial size

250 μg

Concentration

1mg/ml

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

HEK293 cells were transfected with either emply expression vector (pSR? ) or pSRa-Vav2 driving expression of human Vav2. Indicated antibodies (SHD or DPH) were used to immunoprecipitate Vav2 from 300ug protein lysates. Immunoprecipitated activity was identified by immunoblot of complexes using either DPH (upper gel) or SHD (lower gel) antisera at (1:10000). Immunoprecipitation of Endogenous/Exogenous Vav2 from HEK 283 (75 ug lysate per lane). Top gel - i.p. Ab: DPH or SHD preimmune serum (2.5 ul/300ug lysate). DPH or SHD alpha Vav2 antiserum (2.5 ul/300ug lysate). Western Blot Ab: DPH anti Vav2 1:10000. Bottom Gel - i.p. Ab DPH or SHD preimmune sera (2.5 ul/300ug lysate). DPH or SHD anti Vav2 antisera (2.5ul/300ug lysate). Wersterm blot Ab - SHD