GTX127310

antibody from GeneTex

Targeting: TWIST1 ACS3, bHLHa38, BPES2, BPES3, CRS, CRS1, H-twist, SCS, TWIST

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX127310

Provider

GeneTex

Product name

Twist1/2 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

Unveiling the role of microRNA-7 in linking TGF-β-Smad-mediated epithelial-mesenchymal transition with negative regulation of trophoblast invasion.

Shih JC, Lin HH, Hsiao AC, Su YT, Tsai S, Chien CL, Kung HN
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Feb 21;:fj201801898RR

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PEGylated liposome-encapsulated rhenium-188 radiopharmaceutical inhibits proliferation and epithelial-mesenchymal transition of human head and neck cancer cells in vivo with repeated therapy.

Chang CY, Chen CC, Lin LT, Chang CH, Chen LC, Wang HE, Lee TW, Lee YJ
Cell death discovery 2018;4:100

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Upregulation of LGALS1 is associated with oral cancer metastasis.

Li JM, Tseng CW, Lin CC, Law CH, Chien YA, Kuo WH, Chou HC, Wang WC, Chan HL
Therapeutic advances in medical oncology 2018;10:1758835918794622

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Overexpression of CD44 Standard Isoform Upregulates HIF-1α Signaling in Hypoxic Breast Cancer Cells.

Ryu D, Ryoo IG, Kwak MK
Biomolecules & therapeutics 2018 Sep 1;26(5):487-493

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Novel microtubule inhibitor MPT0B098 inhibits hypoxia-induced epithelial-to-mesenchymal transition in head and neck squamous cell carcinoma.

Tsai IT, Kuo CC, Liou JP, Chang JY
Journal of biomedical science 2018 Mar 28;25(1):28

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Activation of fibroblasts by nicotine promotes the epithelial-mesenchymal transition and motility of breast cancer cells.

Chen PC, Lee WY, Ling HH, Cheng CH, Chen KC, Lin CW
Journal of cellular physiology 2018 Jun;233(6):4972-4980

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Corylin Suppresses Hepatocellular Carcinoma Progression via the Inhibition of Epithelial-Mesenchymal Transition, Mediated by Long Noncoding RNA GAS5.

Chen CY, Chen CC, Shieh TM, Hsueh C, Wang SH, Leu YL, Lian JH, Wang TH
International journal of molecular sciences 2018 Jan 27;19(2)

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Elevated G6PD expression contributes to migration and invasion of hepatocellular carcinoma cells by inducing epithelial-mesenchymal transition.

Lu M, Lu L, Dong Q, Yu G, Chen J, Qin L, Wang L, Zhu W, Jia H
Acta biochimica et biophysica Sinica 2018 Apr 1;50(4):370-380

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miR205 inhibits stem cell renewal in SUM159PT breast cancer cells.

Mayoral-Varo V, Calcabrini A, Sánchez-Bailón MP, Martín-Pérez J
PloS one 2017;12(11):e0188637

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Podocalyxin-Like Protein 1 Regulates TAZ Signaling and Stemness Properties in Colon Cancer.

Lee WY, Kuo CC, Lin BX, Cheng CH, Chen KC, Lin CW
International journal of molecular sciences 2017 Sep 23;18(10)

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Targeting LncRNA HOTAIR suppresses cancer stemness and metastasis in oral carcinomas stem cells through modulation of EMT.

Lu MY, Liao YW, Chen PY, Hsieh PL, Fang CY, Wu CY, Yen ML, Peng BY, Wang DP, Cheng HC, Wu CZ, Shih YH, Wang DJ, Yu CC, Tsai LL
Oncotarget 2017 Nov 17;8(58):98542-98552

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Downregulation of miR-199a/b-5p is associated with GCNT2 induction upon epithelial-mesenchymal transition in colon cancer.

Chao CC, Wu PH, Huang HC, Chung HY, Chou YC, Cai BH, Kannagi R
FEBS letters 2017 Jul;591(13):1902-1917

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Aqueous extract of Polygonum bistorta modulates proteostasis by ROS-induced ER stress in human hepatoma cells.

Liu YH, Weng YP, Lin HY, Tang SW, Chen CJ, Liang CJ, Ku CY, Lin JY
Scientific reports 2017 Jan 30;7:41437

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Elevation of YAP promotes the epithelial-mesenchymal transition and tumor aggressiveness in colorectal cancer.

Ling HH, Kuo CC, Lin BX, Huang YH, Lin CW
Experimental cell research 2017 Jan 1;350(1):218-225

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Transcription factor SPZ1 promotes TWIST-mediated epithelial-mesenchymal transition and oncogenesis in human liver cancer.

Wang LT, Chiou SS, Chai CY, Hsi E, Chiang CM, Huang SK, Wang SN, Yokoyama KK, Hsu SH
Oncogene 2017 Aug;36(31):4405-4414

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Oxystressed tumor microenvironment potentiates epithelial to mesenchymal transition and alters cellular bioenergetics towards cancer progression.

Sridaran D, Ramamoorthi G, MahaboobKhan R, Kumpati P
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Oct;37(10):13307-13322

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The Breast Cancer Tumor Suppressor TRIM29 Is Expressed via ATM-dependent Signaling in Response to Hypoxia.

Dükel M, Streitfeld WS, Tang TC, Backman LR, Ai L, May WS, Brown KD
The Journal of biological chemistry 2016 Oct 7;291(41):21541-21552

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Overexpression of lipocalin 2 in human cervical cancer enhances tumor invasion.

Chung IH, Wu TI, Liao CJ, Hu JY, Lin YH, Tai PJ, Lai CH, Lin KH
Oncotarget 2016 Mar 8;7(10):11113-26

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Aberrant DNA hypomethylation of miR-196b contributes to migration and invasion of oral cancer.

Hou YY, You JJ, Yang CM, Pan HW, Chen HC, Lee JH, Lin YS, Liou HH, Liu PF, Chi CC, Ger LP, Tsai KW
Oncology letters 2016 Jun;11(6):4013-4021

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NKX6.1 functions as a metastatic suppressor through epigenetic regulation of the epithelial-mesenchymal transition.

Li HJ, Yu PN, Huang KY, Su HY, Hsiao TH, Chang CP, Yu MH, Lin YW
Oncogene 2016 Apr 28;35(17):2266-78

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MiR-206 suppresses epithelial mesenchymal transition by targeting TGF-β signaling in estrogen receptor positive breast cancer cells.

Yin K, Yin W, Wang Y, Zhou L, Liu Y, Yang G, Wang J, Lu J
Oncotarget 2016 Apr 26;7(17):24537-48

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Cathelicidin suppresses colon cancer development by inhibition of cancer associated fibroblasts.

Cheng M, Ho S, Yoo JH, Tran DH, Bakirtzi K, Su B, Tran DH, Kubota Y, Ichikawa R, Koon HW
Clinical and experimental gastroenterology 2015;8:13-29

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Targeting annexin A2 reduces tumorigenesis and therapeutic resistance of nasopharyngeal carcinoma.

Chen CY, Lin YS, Chen CL, Chao PZ, Chiou JF, Kuo CC, Lee FP, Lin YF, Sung YH, Lin YT, Li CF, Chen YJ, Chen CH
Oncotarget 2015 Sep 29;6(29):26946-59

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Long non-coding RNA AOC4P suppresses hepatocellular carcinoma metastasis by enhancing vimentin degradation and inhibiting epithelial-mesenchymal transition.

Wang TH, Lin YS, Chen Y, Yeh CT, Huang YL, Hsieh TH, Shieh TM, Hsueh C, Chen TC
Oncotarget 2015 Sep 15;6(27):23342-57

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PGRMC1 contributes to doxorubicin-induced chemoresistance in MES-SA uterine sarcoma.

Lin ST, May EW, Chang JF, Hu RY, Wang LH, Chan HL
Cellular and molecular life sciences : CMLS 2015 Jun;72(12):2395-409

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The reciprocal regulation loop of Notch2 pathway and miR-23b in controlling gastric carcinogenesis.

Huang TT, Ping YH, Wang AM, Ke CC, Fang WL, Huang KH, Lee HC, Chi CW, Yeh TS
Oncotarget 2015 Jul 20;6(20):18012-26

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Identification of thiostrepton as a novel therapeutic agent that targets human colon cancer stem cells.

Ju SY, Huang CY, Huang WC, Su Y
Cell death & disease 2015 Jul 2;6(7):e1801

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Antroquinonol from Antrodia Camphorata suppresses breast tumor migration/invasion through inhibiting ERK-AP-1- and AKT-NF-κB-dependent MMP-9 and epithelial-mesenchymal transition expressions.

Lee WT, Lee TH, Cheng CH, Chen KC, Chen YC, Lin CW
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2015 Apr;78:33-41

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: Raji B: THP-1 C: HL-60 12% SDS PAGE GTX127310 diluted at 1:1000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: Twist1 transfected 293T lysates 12% SDS PAGE GTX127310 diluted at 1:5000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Twist1/2 antibody detects Twist1/2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Twist1/2 antibody (GTX127310) diluted at 1:1000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

TWIST antibody detects TWIST protein by western blot analysis. Non-transfected (-) and TWIST1-transfected (+, ) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with TWIST antibody (GTX127310) at a dilution of 1:5000.

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Twist1/2 antibody detects Twist1 protein by western blot analysis. Non-transfected (-) and Twist1-transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Twist1/2 antibody (GTX127310) diluted at 1:5000.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Twist1/2 antibody detects Twist1/2 protein at nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Twist1/2 antibody (GTX127310) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Twist1/2 antibody detects Twist1/2 protein at nucleus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Twist1/2 antibody (GTX127310) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Twist1/2 antibody detects Twist1/2 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. Twist1/2 antibody (GTX127310) dilution: 1:500.