ALX-804-141-C100

antibody from Enzo Life Sciences

Targeting: TNFSF13 APRIL, CD256

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

ALX-804-141-C100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ALX-804-141-C100, RRID:AB_2050735

Product name

APRIL monoclonal antibody (Sacha-1)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Reactivity

Human, Mouse

Host

Rat

Isotype

IgG

Antibody clone number

Sacha-1

Vial size

100 μg

Storage

+4°C

Handling

Avoid freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

MAb to APRIL (Sacha-1) (Prod. No. ALX-804-141) detects membrane-bound human APRIL by FACS. Method: HEK 293TÊ cells (5x10E5) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of a non-cleavable mouse APRIL (thick line). Cells were incubated on ice for 30 min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1µg/ml of MAb to APRIL (Sacha-1) (Prod. No. ALX-804-141).Ê After washing in FACS buffer, PE-conjugated antibody to rat IgG (Prod. No. ALX-211-053) was added. Cells were incubated on ice for 30 min., washed and then analyzed by flow cytometry.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Immunostaining of HEK-293T cells transfected with a murine APRIL expression plasmid (left panels), or mock transfected (right panel). Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with acetone. Slides were blocked with IgG, and incubated for 1 hour with 5µg/ml Sacha-1 or control murine IgG (isotype control) in 1%BSA / 1x PBS for 1 hour. After washes wish PBS, samples were incubated with the secondary Ab for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC.