Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [2]
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Validation data
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- Product number
- ALX-804-141-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-141-C100, RRID:AB_2050735
- Product name
- APRIL monoclonal antibody (Sacha-1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- Sacha-1
- Vial size
- 100 μg
- Storage
- +4°C
- Handling
- Avoid freeze/thaw cycles.
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- MAb to APRIL (Sacha-1) (Prod. No. ALX-804-141) detects membrane-bound human APRIL by FACS. Method: HEK 293TÊ cells (5x10E5) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of a non-cleavable mouse APRIL (thick line). Cells were incubated on ice for 30 min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1µg/ml of MAb to APRIL (Sacha-1) (Prod. No. ALX-804-141).Ê After washing in FACS buffer, PE-conjugated antibody to rat IgG (Prod. No. ALX-211-053) was added. Cells were incubated on ice for 30 min., washed and then analyzed by flow cytometry.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunostaining of HEK-293T cells transfected with a murine APRIL expression plasmid (left panels), or mock transfected (right panel). Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with acetone. Slides were blocked with IgG, and incubated for 1 hour with 5µg/ml Sacha-1 or control murine IgG (isotype control) in 1%BSA / 1x PBS for 1 hour. After washes wish PBS, samples were incubated with the secondary Ab for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC.