Antibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [3]
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Validation data
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- Product number
- ALX-804-148-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-148-C100, RRID:AB_2050730
- Product name
- APRIL (human) monoclonal antibody (Aprily-1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- Aprily-1
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references BAFF production by antigen-presenting cells provides T cell co-stimulation.
Huard B, Arlettaz L, Ambrose C, Kindler V, Mauri D, Roosnek E, Tschopp J, Schneider P, French LE
International immunology 2004 Mar;16(3):467-75
International immunology 2004 Mar;16(3):467-75
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
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- Experimental details
- Western blot of total cell extracts from HEK 293 cells transfected with the indicated expression vector. Aprily-1 reacts specifically with human APRIL. Method: 10µg of protein was applied to the gel. Revealed with Aprily-1 (1µg/ml) and HRP-coupled anti-mouse secondary antibodyÊ(1:4'000).Note: The human APRIL construct used in this experiment is an uncleavable fusion protein between human BAFF (aa 1-132) and human APRIL (aa 93-233).
- Submitted by
- Enzo Life Sciences (provider)
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- Experimental details
- ÊDetection of endogenous human APRIL with MAb to APRIL (Aprily-1) after IP with recombinant human BCMA:Fc. Method: Cell supernatants were first precleared with protein-A sepharose and then subjected to immunoprecipitation with rhBCMA:Fc (Prod. No. ALX-522-026) and protein-A sepharose. Beads were washed twice with PBS.ÊImmunoprecipitated materials were eluted with 2.5mM glycine pH 2.5 for 10 min., neutralized with Tris buffer and run on an SDS-PAGE gel and analyzed with 2µg/ml Aprily-1 by Western blot.ÊNote: In some migrations endogenous soluble APRIL resolved as a doublet around 20kDa (dendritic cells: DC). MegaAPRILª, Soluble (human) (rec.) (Prod. No. ALX-522-035)Êserved as a positive control.
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- Enzo Life Sciences (provider)
- Main image
- Experimental details
- FACS analysis of cells with MAb to hAPRIL (Aprily-1). Method: HEK 293Ê cells were mock transfected or transfected with an expression plasmid enabling surface expression of a non-cleavable human APRIL. Cells (5 x 10E5) were incubated on ice for 30 min in 50 µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 10µg/ml of Aprily-1 antibody. After washing in FACS buffer, FITC-conjugated antibody to mouse IgG was added. Cells were incubated on ice for 30 min., washed and analyzed by flow cytometry.