PA5-64189

antibody from Invitrogen Antibodies

Targeting: BHLHE22 Beta3, BHLHB5, CAGL85, TNRC20

Western blot

Antibody data

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Validation data

Product number: PA5-64189 - Provider product page
Provider: Invitrogen Antibodies
Product name: BHLHB5 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Immunogen sequence: EDDLFLHKSL SASTSKRLEA AFRSTPPGMD LSLAPPPRER PASSSSSPLG CFEPADPEGA GLLLPPP Highest antigen sequence identity to the following orthologs: Mouse - 91%, Rat - 93%.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.16 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

BHLHE22 protein structure - PA5-64189 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of BHLHB5 was performed using log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with BHLHB5 Polyclonal Antibody (Product # PA5-64189) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents cells with no primary antibody to assess background. Panel f shows Jurkat cells with low expression of BHLHB5 as per gene expression databases. The images were captured at 60X magnification..

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of BHLHB5 was performed using log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with BHLHB5 Polyclonal Antibody (Product # PA5-64189) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents cells with no primary antibody to assess background. Panel f shows Jurkat cells with low expression of BHLHB5 as per gene expression databases. The images were captured at 60X magnification..

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of BHLHB5 in human cerebellum, colon, liver and testis using BHLHB5 Polyclonal Antibody (Product # PA5-64189) (A) shows similar protein distribution across tissues to an independent BHLHB5 Polyclonal Antibody (B).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of BHLHB5 in human seminal vesicle using BHLHB5 Polyclonal Antibody (Product # PA5-64189) shows moderate nuclear positivity in glandular cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of BHLHB5 in human skeletal muscle using BHLHB5 Polyclonal Antibody (Product # PA5-64189) shows no positivity in myocytes as expected.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of BHLHB5 in human liver using BHLHB5 Polyclonal Antibody (Product # PA5-64189).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of BHLHB5 in human colon using BHLHB5 Polyclonal Antibody (Product # PA5-64189).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of BHLHB5 in human cerebellum using BHLHB5 Polyclonal Antibody (Product # PA5-64189) shows strong nuclear positivity in cells in molecular layer.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of BHLHB5 in human testis using BHLHB5 Polyclonal Antibody (Product # PA5-64189).