PA5-64326

antibody from Invitrogen Antibodies

Targeting: BHLHE22 Beta3, BHLHB5, CAGL85, TNRC20

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-64326

Provider

Invitrogen Antibodies

Product name

BHLHB5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: GLLVGSAGVG GDPSLSSLPA GAALCLKYGE SASRGSVAES SGGEQSPDDD SDGRCELVLR AGV

Concentration

0.1 mg/mL

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of BHLHB5 was performed using log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with BHLHB5 Polyclonal Antibody (Product # PA5-64326) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents cells with no primary antibody to assess background. Panel f shows Jurkat cells with low expression of BHLHB5 as per gene expression databases. The images were captured at 60X magnification..

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of BHLHB5 in human liver using BHLHB5 Polyclonal Antibody (Product # PA5-64326).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of BHLHB5 in human cerebellum using BHLHB5 Polyclonal Antibody (Product # PA5-64326).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of BHLHB5 in human testis using BHLHB5 Polyclonal Antibody (Product # PA5-64326).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of BHLHB5 in human colon using BHLHB5 Polyclonal Antibody (Product # PA5-64326).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of BHLHB5 in human cerebellum, colon, liver and testis using BHLHB5 Polyclonal Antibody (Product # PA5-64326) (A) shows similar protein distribution across tissues to an independent BHLHB5 Polyclonal Antibody (B).