GTX48598

antibody from GeneTex

Targeting: MACC1 7A5, SH3BP4L

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX48598

Provider

GeneTex

Proper citation

GeneTex Cat#GTX48598, RRID:AB_10620775

Product name

Macc1 antibody

Antibody type

Polyclonal

Host

Rabbit

Vial size

50 µg

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western Blot: Detection of endogenous PTEN-P1 in whole cell lysates from human derived cell lines HeLa (Lanes 4), HEK293 (Lanes 5) and MCF7 (Lanes 6). The band at ~55 kDa (arrowhead) corresponds to PTEN-P1. Lanes 1-3 were show the results of staining after the antibody was first pre-incubated with the immunizing peptide. The identity of lower molecular weight bands in lane 4 is unknown. Briefly, each lane contains approximately 35 ug of lysate. Primary antibody was used at a 1:500 dilution in 5% BLOTTO in PBS reacted overnight at 4 degrees C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the OdysseyInfrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Validation comment

WB