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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PA5-110238 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PTH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LTKSDGKSVK KRSVSEIQLM HNLGKHLNSM ERVEWLRKKL QDVHNFVALG APLAPRDAGS QRPRKKEDNV LVESHEKSLG EADKADVNVL TKAKSQ
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.99 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PTH in extracts of various cell lines. Samples were incubated with PTH Polyclonal antibody (Product # PA5-110238) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of PTH in U-2OS cells. Samples were incubated in polyclonal PTH antibody (Product # PA5-110238) using a dilution of 1:50, followed by DAPI.
