Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-22213 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal ABCB5 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Cellular and molecular assessment of rose bengal photodynamic antimicrobial therapy on keratocytes, corneal endothelium and limbal stem cell niche.
Naranjo A, Pelaez D, Arrieta E, Salero-Coca E, Martinez JD, Sabater AL, Amescua G, Parel JM
Experimental eye research 2019 Nov;188:107808
Experimental eye research 2019 Nov;188:107808
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ABCB5 Antibody (5H3C6) [NBP2-22213] - Analysis using ABCB5 mouse mAb against A431 (1) cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ABCB5 Antibody (5H3C6) [NBP2-22213] - Analysis using ABCB5 mAb against human ABCB5 recombinant protein. (Expected MW is 47 kDa)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: ABCB5 Antibody (5H3C6) [NBP2-22213] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ABCB5 Antibody (5H3C6) [NBP2-22213] - Analysis of paraffin-embedded liver cancer tissues using ABCB5 mouse mAb with DAB staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ABCB5 Antibody (5H3C6) [NBP2-22213] - Analysis of paraffin-embedded breast cancer tissues using ABCB5 mouse mAb with DAB staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: ABCB5 Antibody (5H3C6) [NBP2-22213] - Analysis of HepG2 cells using ABCB5 mouse mAb (green) and negative control (purple).