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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Flow cytometry [2]
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- Product number
- AP6122a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP6122a, RRID:AB_2220819
- Product name
- ABCB5 Antibody (N-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Side population cells from human melanoma tumors reveal diverse mechanisms for chemoresistance.
ABCB5 identifies a therapy-refractory tumor cell population in colorectal cancer patients.
Melanoma spheroids grown under neural crest cell conditions are highly plastic migratory/invasive tumor cells endowed with immunomodulator function.
Luo Y, Ellis LZ, Dallaglio K, Takeda M, Robinson WA, Robinson SE, Liu W, Lewis KD, McCarter MD, Gonzalez R, Norris DA, Roop DR, Spritz RA, Ahn NG, Fujita M
The Journal of investigative dermatology 2012 Oct;132(10):2440-50
The Journal of investigative dermatology 2012 Oct;132(10):2440-50
ABCB5 identifies a therapy-refractory tumor cell population in colorectal cancer patients.
Wilson BJ, Schatton T, Zhan Q, Gasser M, Ma J, Saab KR, Schanche R, Waaga-Gasser AM, Gold JS, Huang Q, Murphy GF, Frank MH, Frank NY
Cancer research 2011 Aug 1;71(15):5307-16
Cancer research 2011 Aug 1;71(15):5307-16
Melanoma spheroids grown under neural crest cell conditions are highly plastic migratory/invasive tumor cells endowed with immunomodulator function.
Ramgolam K, Lauriol J, Lalou C, Lauden L, Michel L, de la Grange P, Khatib AM, Aoudjit F, Charron D, Alcaide-Loridan C, Al-Daccak R
PloS one 2011 Apr 15;6(4):e18784
PloS one 2011 Apr 15;6(4):e18784
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Western blot analysis of ABCB5 Antibody (N-term) (Cat.#AP6122a) in A375, K562, A2058 and HL-60 cell line lysates (35ug/lane). ABCB5 (arrow) was detected using the purified Pab."
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Flow Cytometry using ABCB5 Antibody (N-Term) Cat.# AP6122a on WM852 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit (Jackson ImmunoResearch) as a secondary antibody.?Analysis was done on an FC500 flow cytometer. Data courtesy of Dr. Steve Reuland, University of Colorado, Denver"
- Primary Ab dilution
- 1:10~50
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Flow Cytometry using ABCB5 Antibody (N-Term) Cat.# AP6122a on SkMel-30 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit (Jackson ImmunoResearch) as a secondary antibody.?Analysis was done on an FC500 flow cytometer. Data courtesy of Dr. Steve Reuland, University of Colorado, Denver"
- Primary Ab dilution
- 1:10~50
