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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AP09395PU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09395PU-S, RRID:AB_2035066
- Product name
- anti ABCB5
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to residues corresponding to an internal region of Human ABCB5
- Reactivity
- Human, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µl
- Concentration
- 1.29 mg/ml (AP09395PU-N) / 0.31 mg/ml (AP09395PU-S) - by UV absorbance at 280 nm
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Western blot using affinity purified anti-ABCB5 antibody shows detection of ABCB5 beta in ~12.5 µg of transfected-Hi5 whole cell lysate. No reaction was seen when antibody was pre-incubated with the immunizing peptide (data not shown). A 3-8% Tris-acetate gel was used for separation. The arrowhead corresponds to 117 kDa ABCB5. The membrane was probed with the primary antibody at a 1:10,000 dilution in 5% milk in TBST at 4° C, overnight.
