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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP09395PU-S - Provider product page
- Provider
- OriGene
- Product name
- ABCB5 rabbit polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- ABCB5 rabbit polyclonal antibody, Aff - Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ABCB5
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 25 µl
- Concentration
- 1.29 mg/ml (AP09395PU-N) / 0.31 mg/ml (AP0939
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot using affinity purified anti-ABCB5 antibody shows detection of ABCB5 beta in ~12.5 ug of transfected-Hi5 whole cell lysate. No reaction was seen when antibody was pre-incubated with the immunizing peptide (data not shown). A 3-8% Tris-acetate gel was used for separation. The arrowhead corresponds to 117 kDa ABCB5. The membrane was probed with the primary antibody at a 1:10,000 dilution in 5% milk in TBST at 4¡ã C, overnight.
- Validation comment
- WB
