A02979

antibody from Boster Biological Technology

Targeting: ABCB5 ABCB5alpha, ABCB5beta, EST422562

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A02979

Provider

Boster Biological Technology

Product name

Anti-ABCB5 Antibody

Antibody type

Polyclonal

Description

Rabbit Polyclonal antibody for ABCB5 detection. Tested positive for ELISA,Immunohistochemistry,Western Blot, in human

Reactivity

Human

Host

Rabbit

Vial size

100ug

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

Store Anti-ABCB5 at -20° C prior to opening. Aliquot antibody and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of ABCB5 using anti-ABCB5 antibody (A02979).ABCB5 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ABCB5 Antibody (A02979) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image