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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- GTX85643 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX85643, RRID:AB_10732424
- Product name
- ABCB5 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot of K562 cell line lysates (35 ug/lane).
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Human Breast (formalin-fixed, paraffin-embedded) stained with ABCB5 antibody (GTX85643) followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow Cytometry using ABCB5 Antibody (N-Term) GTX85643 on SkMel-30 cell line. Live cell staining utilized PE-conjμgated goat anti-rabbit as a secondary antibody. Analysis was done on an FC500 flow cytometer
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow Cytometry using ABCB5 Antibody (N-Term) GTX85643 on WM852 cell line. Live cell staining utilized PE-conjμgated goat anti-rabbit as a secondary antibody. Analysis was done on an FC500 flow cytometer.
