Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- HPA014967 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA014967, RRID:AB_1854503
- Product name
- Anti-SLC12A1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human SLC12A1, Gene description: solute carrier family 12 (sodium/potassium/chloride transporter), member 1, Alternative Gene Names: NKCC2, Validated applications: IHC, ICC, Uniprot ID: Q13621, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Soft, Dynamic Hydrogel Confinement Improves Kidney Organoid Lumen Morphology and Reduces Epithelial–Mesenchymal Transition in Culture
Activation of the calcium sensing receptor increases claudin‐14 expression via a PLC ‐p38‐Sp1 pathway
Single cell transcriptional and chromatin accessibility profiling redefine cellular heterogeneity in the adult human kidney
H+-ATPase B1 subunit localizes to thick ascending limb and distal convoluted tubule of rodent and human kidney
Ruiter F, Morgan F, Roumans N, Schumacher A, Slaats G, Moroni L, LaPointe V, Baker M
Advanced Science 2022;9(20)
Advanced Science 2022;9(20)
Activation of the calcium sensing receptor increases claudin‐14 expression via a PLC ‐p38‐Sp1 pathway
Lee J, Alzamil J, Rehman S, Pan W, Dimke H, Alexander R
The FASEB Journal 2021;35(11)
The FASEB Journal 2021;35(11)
Single cell transcriptional and chromatin accessibility profiling redefine cellular heterogeneity in the adult human kidney
Muto Y, Wilson P, Ledru N, Wu H, Dimke H, Waikar S, Humphreys B
Nature Communications 2021;12(1)
Nature Communications 2021;12(1)
H+-ATPase B1 subunit localizes to thick ascending limb and distal convoluted tubule of rodent and human kidney
Frische S, Chambrey R, Trepiccione F, Zamani R, Marcussen N, Alexander R, Skjødt K, Svenningsen P, Dimke H
American Journal of Physiology-Renal Physiology 2018;315(3):F429-F444
American Journal of Physiology-Renal Physiology 2018;315(3):F429-F444
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Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to nucleus & vesicles.
- Sample type
- Human
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human kidney and pancreas tissues using HPA014967 antibody. Corresponding SLC12A1 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol