BM4008

antibody from OriGene

Targeting: ADGRE1 EMR1, TM7LN3

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Immunoelectron microscopy (Data presented on provider website)

Antibody Data

Product number

BM4008

Provider

OriGene

Product name

Adgre1 rat monoclonal antibody, clone Cl:A3-1, Purified

Antibody type

Monoclonal

Description

Adgre1 rat monoclonal antibody, clone Cl:A3-1, Purified

Host

Rat

Conjugate

Unconjugated

Epitope

Adgre1

Isotype

IgG

Antibody clone number

Cl:A3-1

Vial size

500 µg

Concentration

1.0 mg/ml

References

Submitted references

Monocytes/macrophages cooperate with progenitor cells during neovascularization and tissue repair: conversion of cell columns into fibrovascular bundles.

Anghelina M, Krishnan P, Moldovan L, Moldovan NI
The American journal of pathology 2006 Feb;168(2):529-41

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Mouse?anti-Macrophage F4/80 antigen antibody F (5 ug/ml)?on Raw 204.4 cells. Cells were fixed in 1% PFA, permeabilized in 0.25%Triton X 100 in PBS, blocked in 1% BSA in PBS.

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Mouse Spleen Sections stained with Rat anti Mouse F4/80 Antibody.

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Frozen Mouse Spleen stained with Rat anti Mouse F4/80 Antibody No using SP1020HRP as the secondary

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Staining of Mouse peritoneal macrophages with Rat Anti Mouse F4/80 Antigen .

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Mouse?anti-Macrophage F4/80 antigen antibody F?on thioglycollate elicited mouse peritoneal macrophages. Purple: F; Green: Isotype control SM19FS; Percentages reflect % positive after subtraction of negative control, using M1 marker.

Validation comment

FC