60027

antibody from STEMCELL Technologies Inc

Targeting: ADGRE1 EMR1, TM7LN3

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

60027

Provider

STEMCELL Technologies Inc

Product name

Anti-Mouse F4/80 Antibody, Clone BM8

Antibody type

Monoclonal

Description

The BM8 antibody reacts with the F4/80 antigen (also termed Ly-71 in mouse and EMR-1 in human), an ~160 kDa transmembrane glycoprotein belonging to the EGF-TM7 family of G-protein-coupled receptors. F4/80 is considered a marker of choice for the identification of mature tissue macrophages, being broadly but variably expressed by this cell type in the liver (Kupffer cells), skin (Langerhans cells), bone marrow stroma, pancreas, thymus, spleen (red pulp), lung, and other tissues. It is also expressed by circulating monocytes, eosinophils and a subset of dendritic cells. F4/80 expression levels increase following activation of macrophages. F4/80 is reportedly the only macrophage marker suitable for distinguishing destructive from non-destructive inflammatory processes in the pancreas. The protein is thought to play a role in the generation of CD8+ regulatory T cells involved in immune tolerance. This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse Monocyte Enrichment Kit (Catalog #19761) and EasySep™ Mouse CD11b Positive Selection Kit (Catalog #18770).

Reactivity

Human, Mouse

Isotype

IgG

Vial size

500 µg

Storage

2 - 8°C