Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-21399 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EMR1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, Raji, K562, C8D30, NIH-3T3, Raw264.7, C2C12, rat liver.
- Concentration
- 0.23 mg/mL
Submitted references Prophylactic treatment with CN-105 improves functional outcomes in a murine model of closed head injury.
Cardioprotective actions of relaxin.
Relaxin reverses inflammatory and immune signals in aged hearts.
Van Wyck D, Kolls BJ, Wang H, Cantillana V, Maughan M, Laskowitz DT
Experimental brain research 2022 Sep;240(9):2413-2423
Experimental brain research 2022 Sep;240(9):2413-2423
Cardioprotective actions of relaxin.
Martin B, Romero G, Salama G
Molecular and cellular endocrinology 2019 May 1;487:45-53
Molecular and cellular endocrinology 2019 May 1;487:45-53
Relaxin reverses inflammatory and immune signals in aged hearts.
Martin B, Gabris-Weber BA, Reddy R, Romero G, Chattopadhyay A, Salama G
PloS one 2018;13(1):e0190935
PloS one 2018;13(1):e0190935
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EMR1 using A) 30 µg Jurkat whole cell lysate (B) 30 µg Raji whole cell lysate and C) 30 µg K562 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an EMR1 polyclonal antibody (Product # PA5-21399) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using EMR1 Polyclonal Antibody (Product # PA5-21399). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EMR1 Polyclonal Antibody (Product # PA5-21399) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- EMR1 antibody [C2C3], C-term detects EMR1 protein by western blot analysis. A. 50 µg rat liver lysate/extract.7.5% SDS-PAGE. EMR1 antibody [C2C3], C-term EMR1 Polyclonal Antibody (Product # PA5-21399) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of F4/80 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a F4/80 Polyclonal Antibody (Product # PA5-21399) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using EMR1 (Product # PA5-21399) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
