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LearnR32555
antibody from NSJ Bioreagents
Targeting: PSMA1
HC2, MGC14542, MGC14575, MGC14751, MGC1667, MGC21459, MGC22853, MGC23915, NU, PROS30
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- R32555 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PSMA1 Antibody / Proteasome 20S alpha 1
- Antibody type
- Polyclonal
- Description
- This highly specific PSMA1 antibody is suitable for use in Western blot/Immunohistochemistry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the PSMA1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat testis, 2) mouse spleen and 3) human HepG2 lysate with PSMA1 antibody at 0.5ug/ml. Predicted/observed molecular weight ~30 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human MCF7, 2) human PC-3, 3) human HepG2, 4) human Jurkat, 5) rat brain, 6) rat liver, 7) mouse brain and 8) mouse liver tissue lysate with PSMA1 antibody. Predicted molecular weight ~30 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human intestine cancer tissue with PSAT1 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human rectum adenocarcinoma tissue with PSMA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human lung cancer tissue with PSMA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
