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LearnLS-C679225
antibody from LSBio
Targeting: PSMA1
HC2, MGC14542, MGC14575, MGC14751, MGC1667, MGC21459, MGC22853, MGC23915, NU, PROS30
Western blot
ELISA
Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [2]
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Validation data
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- Product number
- LS-C679225 - Provider product page
- Provider
- LSBio
- Product name
- PSMA1 Antibody LS-C679225
- Antibody type
- Polyclonal
- Description
- Protein G purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot Positive WB detected in:Hela whole cell lysate,PC3 whole cell lysate,K562 whole cell lysate All Lanes:PSMA1 antibody at 3.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30,31 KDa Observed band size: 30 KDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating PSMA1 in K562 whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of product in K562 whole cell lysate.For western blotting,a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000) Lane 2: product (8µg) + K562 whole cell lysate (500µg) Lane 3: K562 whole cell lysate (10µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating PSMA1 in K562 whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of product in K562 whole cell lysate.For western blotting,a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000) Lane 2: product (8µg) + K562 whole cell lysate (500µg) Lane 3: K562 whole cell lysate (10µg)
