CF502086

antibody from Invitrogen Antibodies

Targeting: GRHPR GLXR, PH2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

CF502086

Provider

Invitrogen Antibodies

Product name

GRHPR Monoclonal Antibody (OTI1A9), TrueMAB™

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI1A9

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRHPR (RC200963, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-GRHPR. Positive lysates LY415912 (100 µg) and LC415912 (20 µg) can be purchased separately from OriGene.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRHPR (RC200963, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-GRHPR. Positive lysates LY415912 (100 µg) and LC415912 (20 µg) can be purchased separately from OriGene.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-GRHPR monoclonal antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Equivalent amounts of cell lysates (10 µg per lane) of wild-type 293T cells (WT, Cat# LC810293T) and GRHPR-Knockout 293T cells (KO, Cat# LC841943) were separated by SDS-PAGE and immunoblotted with anti-GRHPR monoclonal antibody TA502086(1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GRHPR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502086)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human Kidney tissue within the normal limits using anti-GRHPR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502086)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GRHPR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502086)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human liver tissue within the normal limits using anti-GRHPR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502086)

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells transfected with either RC200963 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GRHPR antibody (TA502086), and then analyzed by flow cytometry.