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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA322670 - Provider product page
- Provider
- OriGene
- Product name
- Anti-AKAP12 Rabbit Polyclonal Antibody
- Antibody type
- Polyclonal
- Description
- Anti-AKAP12 Rabbit Polyclonal Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- AKAP12
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- 1.2 mg/ml
Submitted references CD44 is required for the pathogenesis of experimental crescentic glomerulonephritis and collapsing focal segmental glomerulosclerosis.
Eymael J, Sharma S, Loeven MA, Wetzels JF, Mooren F, Florquin S, Deegens JK, Willemsen BK, Sharma V, van Kuppevelt TH, Bakker MA, Ostendorf T, Moeller MJ, Dijkman HB, Smeets B, van der Vlag J
Kidney international 2018 Mar;93(3):626-642
Kidney international 2018 Mar;93(3):626-642
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Predicted band size: 191 kDa. Positive control: HT-29 cell lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 40 seconds)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Predicted cell location: Cytoplasm . Positive control: Human brain tissue . Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using AKAP12 antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x200)
- Validation comment
- IHC
