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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [6]
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- Product number
- PA5-52281 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AKAP12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: QRAEAERPEE QAEASGLKKE TDVVLKVDAQ EAKTEPFTQG KVVGQTTPES FEKAPQVTES IESSELVTTC QAETLAGVKS QEMVMEQAIP PDSVETPTDS ETDGST
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AKAP12 in human cell lines U-251MG and MCF-7 using a AKAP12 Polyclonal Antibody (Product # PA5-52281). Corresponding AKAP12 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of AKAP12 in human cell line U-251 MG shows positivity in plasma membrane & cytoplasm. Samples were probed using an AKAP12 Polyclonal Antibody (Product # PA5-52281).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of AKAP12 in human cell line U-251 MG using an AKAP12 Polyclonal Antibody (Product # PA5-52281) shows localization to plasma membrane and cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of AKAP12 in human kidney using AKAP12 Polyclonal Antibody (Product # PA5-52281).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of AKAP12 in human testis and skeletal muscle tissues using AKAP12 Polyclonal Antibody (Product # PA5-52281). Corresponding AKAP12 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of AKAP12 in human kidney, liver, skeletal muscle and testis using AKAP12 Polyclonal Antibody (Product # PA5-52281) (A) shows similar protein distribution across tissues to an independent AKAP12 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of AKAP12 in human testis using AKAP12 Polyclonal Antibody (Product # PA5-52281) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of AKAP12 in human skeletal muscle using AKAP12 Polyclonal Antibody (Product # PA5-52281) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of AKAP12 in human liver using AKAP12 Polyclonal Antibody (Product # PA5-52281).
