Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [6]
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Validation data
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- Product number
- PA5-63271 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AKAP12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SKDMSEASEK TMTVEVEGST VNDQQLEEVV LPSEEEGGGA GTKSVPEDDG HALLAERIEK SLVEPKEDEK GDDVDDPENQ NSALADTDAS GGLTKESPDT NGPKQKEKED AQEVELQEGK VHSESDKAIT PQAQEELQKQ ERESAKS Highest antigen sequence identity to the following orthologs: Mouse - 30%, Rat - 34%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of AKAP12 in human cell line U-251 MG shows positivity in plasma membrane & cytoplasm. Samples were probed using an AKAP12 Polyclonal Antibody (Product # PA5-63271).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of AKAP12 in human cell line U-251 MG using an AKAP12 Polyclonal Antibody (Product # PA5-63271) shows localization to plasma membrane and cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of AKAP12 in human liver using AKAP12 Polyclonal Antibody (Product # PA5-63271).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of AKAP12 in human testis tissue shows strong cytoplasmic positivity in cells n seminiferus ducts. Samples were probed using an AKAP12 Polyclonal Antibody (Product # PA5-63271).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of AKAP12 in human kidney using AKAP12 Polyclonal Antibody (Product # PA5-63271).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of AKAP12 in human testis and skeletal muscle tissues using AKAP12 Polyclonal Antibody (Product # PA5-63271). Corresponding AKAP12 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of AKAP12 in human kidney, liver, skeletal muscle and testis using AKAP12 Polyclonal Antibody (Product # PA5-63271) (A) shows similar protein distribution across tissues to an independent AKAP12 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of AKAP12 in human skeletal muscle using AKAP12 Polyclonal Antibody (Product # PA5-63271) shows low expression as expected.