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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF550 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat Agrin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects rat Agrin in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant rat (rr) GDNF, rrIL-1 alpha , and rrL-Selectin is observed.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P25304- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Agrin, a novel basement membrane component in human and rat liver, accumulates in cirrhosis and hepatocellular carcinoma.
Neurexins induce differentiation of GABA and glutamate postsynaptic specializations via neuroligins.
Tátrai P, Dudás J, Batmunkh E, Máthé M, Zalatnai A, Schaff Z, Ramadori G, Kovalszky I
Laboratory investigation; a journal of technical methods and pathology 2006 Nov;86(11):1149-60
Laboratory investigation; a journal of technical methods and pathology 2006 Nov;86(11):1149-60
Neurexins induce differentiation of GABA and glutamate postsynaptic specializations via neuroligins.
Graf ER, Zhang X, Jin SX, Linhoff MW, Craig AM
Cell 2004 Dec 29;119(7):1013-26
Cell 2004 Dec 29;119(7):1013-26
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Agrin in Mouse Kidney. Agrin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Rat Agrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF550) at 0.1 µg/mL overnight at 4 °C. Tissue was stained using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to basement membrane of epithelial cells in tubules and endothelial cells in glomeruli. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Acetylcholine Receptor Clustering Induced by Agrin and Neutralization by Rat Agrin Antibody. Recombinant Rat Agrin (Catalog # 550-AG) induces acetylcholine receptor clustering on chick myotubes in a dose-dependent manner (orange line). Acetylcholine Receptor Clustering elicited by Recom-binant Rat Agrin (0.016 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat Agrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF550). The ND50 is typically 0.001-0.004 µg/mL.
