AP7604b

antibody from Abcepta

Targeting: CSF1R C-FMS, CD115, CSFR, FMS

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP7604b

Provider

Abcepta

Proper citation

Product name

MCSF Receptor (CSF1R) Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Western blot analysis of anti-CSF1R Pab (Cat. #AP7604b) in human placenta. CSF1R (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Western blot analysis of CSF1R (arrow) using rabbit polyclonal CSF1R Antibody (C-term)(Cat.#AP7604b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CSF1R gene (Lane 2) (Origene Technologies).

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CSF1R Antibody (C-term) (Cat.#AP7604b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:10~50

Flow cytometry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

MCSF Receptor (CSF1R) Antibody (C-term) (Cat. #AP7604b) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50