Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB3291-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human M-CSF R/CD115 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human M-CSF R/CD115 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) GM-CSF R alpha or rhGM-CSF R beta is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P07333.2
- Isotype
- IgG
- Antibody clone number
- 61701
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Phenotype of vigilin expressing breast cancer cells binding to the 69 nt 3'UTR element in CSF-1R mRNA.
Receptor-Type Protein-Tyrosine Phosphatase ζ and Colony Stimulating Factor-1 Receptor in the Intestine: Cellular Expression and Cytokine- and Chemokine Responses by Interleukin-34 and Colony Stimulating Factor-1.
Autocrine CSF1R signaling mediates switching between invasion and proliferation downstream of TGFβ in claudin-low breast tumor cells.
Macrophage colony-stimulating factor augments Tie2-expressing monocyte differentiation, angiogenic function, and recruitment in a mouse model of breast cancer.
Derepression of an endogenous long terminal repeat activates the CSF1R proto-oncogene in human lymphoma.
Woo HH, Lee SC, Stoffer JB, Rush D, Chambers SK
Translational oncology 2019 Jan;12(1):106-115
Translational oncology 2019 Jan;12(1):106-115
Receptor-Type Protein-Tyrosine Phosphatase ζ and Colony Stimulating Factor-1 Receptor in the Intestine: Cellular Expression and Cytokine- and Chemokine Responses by Interleukin-34 and Colony Stimulating Factor-1.
Zwicker S, Bureik D, Bosma M, Martinez GL, Almer S, Boström EA
PloS one 2016;11(11):e0167324
PloS one 2016;11(11):e0167324
Autocrine CSF1R signaling mediates switching between invasion and proliferation downstream of TGFβ in claudin-low breast tumor cells.
Patsialou A, Wang Y, Pignatelli J, Chen X, Entenberg D, Oktay M, Condeelis JS
Oncogene 2015 May 21;34(21):2721-31
Oncogene 2015 May 21;34(21):2721-31
Macrophage colony-stimulating factor augments Tie2-expressing monocyte differentiation, angiogenic function, and recruitment in a mouse model of breast cancer.
Forget MA, Voorhees JL, Cole SL, Dakhlallah D, Patterson IL, Gross AC, Moldovan L, Mo X, Evans R, Marsh CB, Eubank TD
PloS one 2014;9(6):e98623
PloS one 2014;9(6):e98623
Derepression of an endogenous long terminal repeat activates the CSF1R proto-oncogene in human lymphoma.
Lamprecht B, Walter K, Kreher S, Kumar R, Hummel M, Lenze D, Köchert K, Bouhlel MA, Richter J, Soler E, Stadhouders R, Jöhrens K, Wurster KD, Callen DF, Harte MF, Giefing M, Barlow R, Stein H, Anagnostopoulos I, Janz M, Cockerill PN, Siebert R, Dörken B, Bonifer C, Mathas S
Nature medicine 2010 May;16(5):571-9, 1p following 579
Nature medicine 2010 May;16(5):571-9, 1p following 579
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human M-CSF R/CD115 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human M-CSF R/CD115 Monoclonal Antibody (Catalog # MAB3291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for M-CSF R/CD115 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- M-CSF R/CD115 Inhibition of M-CSF-dependent Cell Proliferation and Neutralization by Human M-CSF R/CD115 Antibody. Recombinant Human M-CSF R/CD115 Fc Chimera (Catalog # 329-MR) inhibits Recombinant Human M-CSF (Catalog # 216-MC) induced proliferation in the M-NFS-60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human M-CSF R/CD115 (1 ng/mL) activity elicited by Recombinant Human M-CSF R/CD115 Fc Chimera (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human M-CSF R/CD115 Monoclonal Antibody (Catalog # MAB3291). The ND50 is typically 4-16 ng/mL.